Journal: Cardiovascular research

Article Title: Serotonin receptor antagonist inhibits monocrotaline-induced pulmonary hypertension and prolongs survival in rats.

doi: 10.1016/j.cardiores.2003.09.023

Figure Lengend Snippet: Fig. 2. Immunohistochemical analysis showing P-selectin expression and CD45-positive and PCNA-positive leukocytes in the lung tissue and pulmonary vessels. P-selectin is intensely expressed on the endothelium of the pulmonary arteries in the placebo group, and its expression is markedly suppressed in the sarpogrelate-treated group (original magnification 250). The numbers of the CD45-positive and PCNA-positive cells in the lung tissues are significantly increased in the placebo group compared with the normal control group, and these are significantly decreased after sarpogrelate treatment (original magnification 160).

Article Snippet: The remaining tissue sections of the lung were subjected to immunostaining with antibodies against P-selectin (Santa Cruz Biotechnology, Santa Cruz, CA), proliferating cell nuclear antigen (PCNA) (Dako, Osaka, Japan).

Techniques: Immunohistochemical staining, Expressing, Control

Journal: Stem Cells International

Article Title: Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells

doi: 10.1155/2016/1406304

Figure Lengend Snippet: Interaction of LO-EPC with activated HAEC. Surface expression of adhesion molecules in HAEC after stimulation with 0.05 ng/mL TNF α (a). Surface expressions of E-selectin, ICAM-1, and VCAM-1 were quantified using flow cytometry. The graph shows relative Mean Fluorescence Intensity (MFI) normalised to untreated cells and represents the mean ± SE of three experiments. ∗∗ p < 0.01. Activation of HAEC did not increase the interaction with LO-EPC (b). 4 × 10 5 LO-EPC were perfused to 3 × 10 4 HAEC for 4 min and the data represented as mean ± SE of three experiments.

Article Snippet: To study TNF α mediated HAEC activation, control HAEC and HAEC treated with 0.05 ng/ μ L TNF α (R&D System, UK), for 4 hours at 37°C, were collected in 100 μ L 1% bovine serum albumin (BSA, Sigma) in PBS and incubated with fluorescein conjugated anti-human E-selectin antibodies (R&D System), APC conjugated anti-human ICAM-1 (BD), and PE/Cy5 conjugated anti-human VCAM-1 (Bio Legend, UK), together with the respective isotype control antibodies.

Techniques: Expressing, Flow Cytometry, Fluorescence, Activation Assay